Reverse Transcriptase Enzymes
Fortis Life Sciences offers high-quality reverse transcriptases (RTs) that empower the end user to achieve reliable results in their molecular biology application. These essential enzymes enable the synthesis of complementary DNA (cDNA) from RNA templates, allowing for applications such as quantifying gene expression levels, studying RNA structure and function, and developing sensitive diagnostic assays like RT-PCR and RT-LAMP that aid in the detection of RNA viruses.
Related Products: Reverse transcription (RT) kits
Empowering Your RT-PCR, RT-qPCR, & RT-LAMP Assays with Reliable Performance
When you choose Fortis reverse transcriptase products, you're selecting tools that enable you to:
- Focus on Your Science: Our rigorous quality control processes mean you can trust the performance of our enzymes, allowing you to concentrate on your research.
- Maintain Experimental Consistency: Our focus on maintaining lot-to-lot consistency allows you to confidently compare results across experiments and over time.
- Adapt to Various Applications: Whether you're performing RT-PCR, RT-qPCR, or cDNA synthesis, our reverse transcriptase products are tested to ensure reliable performance across a range of techniques.
Our Reverse Transcription Kit Portfolio
RTScript™ Reverse Transcriptase
RTScript™ is an engineered M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase with reduced RNase H activity and increased thermal stability.
Key Features:
- High sensitivity for low-abundance transcripts
- Efficient synthesis of cDNA up to 10 kb in length
- Reduced RNase H activity for improved full-length cDNA synthesis
- Available in 20,000 and 40,000 unit sizes
Applications:
- First-strand cDNA synthesis
- RT-PCR and RT-qPCR
- Gene expression analysis
- miRNA detection and quantification
ActiTemp-50 RTScript™ Reverse Transcriptase
ActiTemp-50 RTScript™ is a thermostable reverse transcriptase designed for challenging templates, providing warm-start functionality to reduce background reverse transcriptase activity.
Key Features:
- Warm-start modification to minimizes background reverse transcription activity at low temperatures (warm-start modification is reversibly inactivated at temperatures ≥50 °C)
- Increased reverse transcription specificity.
- Enhanced thermal stability for improved performance with complex RNA secondary structures
- Efficient cDNA synthesis at elevated temperatures (up to 55°C)
- Reduced RNase H activity
- Available in kit format for 200 and 400 reactions
Applications:
- cDNA synthesis from GC-rich and structurally complex RNA
- High-temperature RT-PCR
- Transcriptome analysis of thermophilic organisms
IsoReverse Transcriptase
IsoReverse Transcriptase is specially formulated for isothermal amplification techniques, particularly RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification).
Key Features:
- Optimized for isothermal amplification conditions
- High efficiency at constant temperatures (60-65°C)
- Compatible with various buffer conditions
- Available in 1,875U and 7,500U sizes
Applications:
- RT-LAMP for rapid RNA detection
- Isothermal diagnostic assays
- Point-of-care testing
Looking for a Bulk Order?
At Fortis, we're dedicated to providing you with the reliable, high-performance enzymes you need to drive your RNA research and diagnostic applications forward with quality products and a collaborative approach.
